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Hypertensive patients have been observed to produce high levels of hydrogen peroxide, superoxide anion, and NO in leukocytes [290,291,292], exhibit elevated lipid peroxidation in erythrocytes, and display elevated concentrations of 8-isoprostanes in plasma [293]

When using Ni-Penta resins, however, most purification protocols require no imidazole in the binding/equilibration buffer, and up to 5 mM in the wash buffer

Cancer Metab 1 , 23 (2013)

Reconstituting Without Letting the Powder Warm to Room Temperature Cold powder dissolves less efficiently and the temperature shock from adding room-temperature BAC water to a cold vial increases stress on the peptide