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Streptolysin O deficiency in Streptococcus pyogenes M1T1 covR/S mutant strain attenuates virulence in in vitro and in vivo infection models

Multiple reports and recent work have repeatedly shown a decreased intake of food in animals fed a KD, indicating a potential association between GDF15 and the management of obesity by the KD [86]

Briefly, cFSP1 N eluted from the Ni-chelating resin was diluted with 0.1 X PBS buffer supplemented with 5% (v/v) glycerol and 5 mM -mercaptoethanol and loaded onto a HiTrap Heparin HP column (GE Healthcare, USA)