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Using codon-randomizing mutagenesis to generate a clone library bearing nearly 80% of all possible amino acid changes, we measured the functional consequences of CBS variation by measuring the effects of selection on allele frequencies during a competitive yeast complementation assay using next-generation sequencing

In particular, hydrogels of different Bloom degrees, ranging from 90 to 300, were prepared

All methods were performed in accordance with the relevant guidelines and regulations, in agreement with the Declaration of Helsinki

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