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Consistently, SureQuantbased targeted mass spectrometry using two unique CEST2.2 peptides (LSTGTIEGR and HGEDFAYAFGTNR) revealed that our transgene could also restore CEST2.2 protein levels when introduced in gas1(fc21) mutants (Fig 1L and Appendix Table S2)

In respect to vesicular release, it appears unlikely that filling of vesicles with ATP by VNUT is regulated by [ATP] c (at least for the physiological concentration range), given that VNUT is an active transporter and [ATP] c is rather high

Levels of evidence and grades of recommendation The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) system (Table 1) was applied to grade the evidence and recommendations

There is abundant evidence of adverse health effects in all of these populations