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After thawing, cells were filtered through EASYstrainer 40 m (Greiner), washed with PBS and cultivated in RPMI-1640 (Gibco) supplemented with 10% fetal bovine serum (FBS-12A, Capricorn Scientific), 200 U/mL penicillin and 200 g/mL streptomycin (Gibco)

These reactive astroglial changes are implicated in senile plaques at the early to moderate stage of AD during mild cognitive impairment and in the late stage of the disease during severe dementia (Verkhratsky et al., 2019a,b)

Abstract HIV-1 is a retrovirus that integrates into host chromatin and can remain transcriptionally quiescent in a pool of immune cells

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