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In MDD, this peripheral-central coupling is likely amplified by BBB dysfunction characterized by increased permeability, neurovascular inflammation, and associations with symptom severity [172, 173] thereby lowering the threshold at which peripheral KP activation translates into central glutamatergic, oxidative, and neuroimmune dysregulation

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Immunoblotting Cells were washed with ice-cold PBS and lysed with RIPA lysis buffer