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Disorders of cobalamin (Vitamin B 12 ) metabolism: Emerging concepts in pathophysiology, diagnosis and treatment

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Additionally, the purity and integrity of Trx1 were confirmed by electroblotting of samples resolved by 10% PAGE using semi-dry transfer on to a nitrocellulose membrane (22 m) and after blocking with 5% BSA in TBS for 30 min at RT, the membrane was immunoprobed with mouse monoclonal anti Trx1 antibody (Abcam Cat# ab16965, RRID:AB_443587