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Cells were then incubated with a primary antibody for 12 h at room temperature, followed by the corresponding fluorophore-conjugated secondary antibody (Alexa Fluor) for 45 min and DAPI staining (1 g ml 1 DAPI

Sustained elevation of IGF-1 is associated, in observational studies of acromegaly (a disease of GH excess), with increased risk of several cancers including colorectal and breast

for mechanistic isolation, the choice depends on which pathway is being studied