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Environ Sci Pollut Res 16:795804 Sengupta D, Ramesh G, Mudalkar S, Kumar KRR, Kirti PB, Reddy AR (2012) Molecular cloning and characterization of -Glutamyl cysteine Synthetase (VrECS) from roots of Vigna radiata (L.) Wilczek under progressive drought stress and recovery

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0.3 million cells per sample were incubated with the complex in wash buffer (PBS with 2% BSA) for 30 min at 4 C followed by incubating with the detection antibody (Supplementary Table 2) for another 30 min at 4 C in the dark after washing the excess amount of the complex