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Importantly, the senescence-like pro-inflammatory signature driven by NFKB1, ETS2, and KLF6 was still observed, likely because it was not specific but rather shared across multiple AM subpopulations

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M1-type macrophages are generated in response to granulocyte macrophage colony stimulating factors (GM-CSF or CSF2) and stimulated by IFN-, lipopolysaccharide, TNF-, which share the same properties of anti-tumor and pro-inflammation