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1b,c and 3k, pancreata were weighed, then mechanically dissociated, followed by digestion in 5 ml of HBSS containing collagenase I (375 U ml 1 ), DNase I (0.15 mg ml 1 ) and Soybean Trypsin inhibitor (Sigma, 0.05 mg ml 1 ) for 30 min at 37 C on a shaker (220 rpm), followed by dissociation with a syringe and needle, filtration through a 70-m strainer to exclude Langerhans islets and red blood cell lysis

Celastrol, a triterpenoid compound derived from Tripterygium wilfordii, a traditional Chinese medicine, demonstrates potential anti-glioma effects

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