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Key regulators of galectin-glycan interactions

Wallach D, Kang TB, Kovalenko A

linear flow rate 250 cm/h Chemical stability stable in 0.1 M acetate pH 4.0, 0.1 M NaOH, 70 % ethanol or 6 M guanidine hydrochloride for two hours at room temperature, not autoclavable * Tested with human Glutathione S-transferase (GST) Recommended flow rates With a bed volume of 1 ml (10 ml) and an inner diameter of 6.6 mm (16 mm) the flow rates for sample loading should be 0.2 1.0 ml/min (0.5 - 5.0 ml/min) and 1.0 ml/min (5.0 ml/min) for washing/elution

This is followed by the second alkylation step, where newly formed sulfhydryl groups are chemically blocked with an alkylating reagent distinct from that used in the first step [e.g., NEM, IAM, 2-chloroacetamide, iodoacetic acid (IAA), 4-vinylpyridine, methanethiosulfonate (MMTS)] (McDonagh et al., 2014)