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In the previously cited clinical study,9 the elderly subjects received 100 mg/kg of both NAC and glycine for 14 days, and their red blood cell total glutathione levels (reduced glutathione plus diglutathione) rose from 1.26 mmol/L at baseline to 2.23 mmol/L, a concentration slightly higher than that measured in young subjects who did not take supplements.9 Moreover, the ratio of reduced glutathione to diglutathione rose from 7.4:1 to 16.1:1, indicative of a substantial improvement in redox status as confirmed by concurrent reductions in plasma markers of oxidative stress

This can result in the removal of an activation peptide and exposes a new N-terminus in the catalytic domain starting with Ile16

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Fahey MC, Cremer PD, Aw ST, Millist L, Todd MJ, White OB, Halmagyi M, Corben LA, Collins V, Churchyard AJ, Tan K, Kowal L, Delatycki MB