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T.OnfrayM

PGC-1alpha is induced by parathyroid hormone and coactivates Nurr1-mediated promoter activity in osteoblasts

Lysates were concentrated to a final concentration of 1 mg ml 1 by boiling the appropriate amount of protein lysate with 2 Laemmli buffer (4% SDS, 20% glycerol, 10% 2-mercaptoethanol and 0.004% bromophenol blue in 0.2 M Tris-HCl, pH 7) at 90 C for 10 min