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In experiments with primary patient-derived cells, responses were measured after 3 days of incubation

FCCP (10 M) was used 30 min prior to TMRE staining as a positive control of mitochondrial membrane potential loss

TEM evaluation further confirmed the existence of ferroptosis in airway epithelial cells in response to CS exposure and in COPD lungs

Vion AC, Perovic T, Petit C, Hollfinger I, Bartels-Klein E, Frampton E, Gordon E, Claesson-Welsh L, Gerhardt H (2021) Endothelial cell orientation and polarity are controlled by shear stress and VEGF through distinct signaling pathways