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The client is observed and timed while rising from an armchair, walking 3 meters, turning, walking back, and sitting down again (Yoo et al., 2021)

Plates were washed with PBS-T and anti-mouse IgM-HRP or anti-mouse IgG-HRP (Sigma A8786 and A3673) was used for detection

Recently, using the genetically encoded fluorescence resonance energy transfer (FRET) lactate sensor Laconic, a lactate gradient from astrocytes to neurons was demonstrated in vivo (Mchler et al., + /K + -ATPase pump coupled to glutamate transport (consuming one ATP) and the glutamine synthetase (consuming another ATP) for the maintenance of the glutamate-glutamine cycle (Pellerin and Magistretti, 1994)

With the recent de-orphaning of SLC22A15 as a second ergothioneine transporter expressed highly in the brain (Reference Yee, Buitrago and Stecula28) , and likelihood of further ergothioneine transporters yet to be characterised (see Fig