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Recent studies indicate that the gut microbiota and its metabolites are crucial for neurodevelopment, neurodegenerative diseases, and other neurological disorders (McCallum and Tropini, 2024)

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The copper component requires attention in dosing calculations excess copper supplementation can cause hepatotoxic effects in some dog breeds, particularly those with hereditary copper storage disorders (Bedlington Terriers, Labrador Retrievers)

the transfection efficiency was verified using PCR and WB (Fig