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Media was replaced on the cells with 10 ml of DMEM media supplemented with 10% FBS, 1% PenStrep, and 0.6 g/ml Puromycin

Methods: Sandwich ELISAs to measure the matricellular fibrosis markers thrombospondin-2 (TSP2) and thrombospondin-4 (TSP4) as well as the metabolism/inflammation/fibrosis related markers insulin-like growth factor binding protein-7 (IGFBP7), CD163 and a disintegrin and metalloprotease thrombospondin like-9 (ADAMTS9) were developed in-house using combinations of recombinantly expressed proteins as well as commercial and self-produced monoclonal and polyclonal antibodies

Hong S, Kim Y, Sung J, Lee H, Heo H, Jeong H, Lee J (2020) Jujube ( Ziziphus jujuba Mill.) protects hepatocytes against alcohol-induced damage through Nrf2 activation

Mecanismo Reset: O peptdeo no atua apenas como tijolo (cobre), mas como o arquiteto, reprogramando o sistema operacional celular via modulao de genes de reparo de DNA e sistema ubiquitina-proteassoma