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Blob picker was used for initial particle selection from 1210 out of a total of 1216 micrographs, followed by several rounds of 2D classification, resulting in the selection of 269,729 particles to generate a good initial model with obvious secondary structure features by ab initio reconstruction

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Thats the core reason the advantages of vitamin B12 injections over oral supplements matter so much for people with absorption difficulties or a more severe deficiency

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