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Description

Trost BM, Van Vranken DL (1996) Asymmetric transition metal-catalyzed allylic alkylations

fermentum A51 were Gram-stained and observed under a microscope (E200, Nikon, Japan)

To charge RHEB with GTPS (Abcam) or GDP (Sigma-Aldrich), RHEB was first diluted in buffer (50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM TCEP, 5 mM EDTA) and then supplemented with GDP or GTPS at a 30-fold molar excess