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Description

Free Radic Biol Med 99:436450

Kinase activity was determined by the addition of 25 M ATP, 3 Ci of [- 32 P]-ATP (SRP-301, Hartmann Analytic), and 100 M of an optimal Abl substrate sequence carrying an N -terminal Biotin (Biotin-GGEAIYAAPFKK-amide) in kinase assay buffer (20 mM Tris-HCl, pH 7.5, 10 mM MgCl 2 ,1 mM DTT) for 15 min at room temperature in a final assay volume of 20 L