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The towns compact size and beautifully preserved architecture make it easy to explore on foot

The final libraries were quantified using a Qubit Fluorometer (Thermo Fisher Scientific) with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific)

The protocol had been implemented with the default parameters except for Force field (CHARMm) and Ligand partial charge Method (Momey-Rone) 31,32

Candidate biochemical markers were selected based on pilot study results and for citations already-outlined in the Section Introduction. Standardized collection methods, protocols, and citations are documented in Sections S4 and S5 in Supplementary Material