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Description

Furthermore, when MAT2A-overexpressing OS cells were treated with the PI3K/AKT pathway inhibitor LY294002, their proliferation, apoptosis, and the expression of key pathway components were markedly altered (Fig

Western blot Cells were collected by trypsinization and resuspended in NETN150 lysis buffer (NaCl 150 mM, NP40 0.5%, Tris 20 mM pH 8.0, EDTA 1 mM) with protease inhibitor cocktail (1:100) (TargetMol, C0001, China)