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5-EU click reaction was performed in buffer containing 100 mM Tris-HCl (pH 8.5), 2 mM CuSO 4 , 100 mM sodium ascorbate, and 5 M Alexa Fluor 488 Azide (Thermo Fisher Scientific, A10266) for 30 min in the dark followed by two washes with 1% (w/v) BSA in PBS

Because the LFCN is purely sensory, any other neurologic abnormalities on exam such as abnormalities in reflexes, strength, gait, or sensation outside the boundaries of the LFCN, indicate a process beyond just the LFCN

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