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Samples were resolved on 4%20% SDS-PAGE gels and transferred to PVDF membranes

[DOI] [Google Scholar] Hindley J

Cell-Based Proteasome-Glo Assays Homogeneous, Luminescent Assays that Measure Protease Activities in Cultured Cells Simple "add-mix-measure" protocol Chymotrypsin-Like, Trypsin-Like and Caspase-Like activity data directly from cellular environment Results within 10-30 minutes after reagent addition Catalog Number: Choose an assay Size Catalog Number: G8660 Catalog Number: G8661 Catalog Number: G8662 Catalog Number: G8760 Catalog Number: G8761 Catalog Number: G8860 Catalog Number: G8861 Catalog Number: G1180 Catalog Number: G1200 Measure Protease Activity in Cultured Cells Homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells for more biologically relevant results

Here are some general guidelines: Mild deficiency: The typical treatment is a 250-500 mcg liquid B12 dose prescribed to patients with the mildest B12 deficiency