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PMC 2770991

The role of bile acids as aggressors in the intestinal cavity has been studied for many years to the point where no disease has been described in which bile acids are the primary initiator of injury

And Ill be very, very, very, very, very (did I say very enough?) clear: this is not prescriptive

Elevating the temperature of the immunofluorescence detection to 60C after paraformaldehyde fixation and before antibody detection elevated the BDBT signal in the eyes of light-adapted flies to the same level as those from dark adapted flies ( It is likely that this change in antigenicity involves changes in the rhabdomeric structure